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human intestinal epithelial cell lines caco 2 cells (ATCC)

Name: human intestinal epithelial cell lines caco 2 cells
Brand: ATCC
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ATCC human intestinal epithelial cell lines caco 2 cells

Cytotoxicity and inhibitory effects on RV replication of different HSP90 inhibitors. ( A ) Plots for cell viability of MA104, <t>Caco-2,</t> and HT-29 cells after treatment with NVP-HSP990, GA, or 17-AAG at indicated concentrations for 24 h. Cell viability was tested using the CCK-8 assay. ( B ) Plots for RV (Wa and SA11 strains) inhibition in MA104, Caco-2, and HT-29 cells after treatment with NVP-HSP990, GA, or 17-AAG at indicated concentrations for 24 h. RV replication was tested by PFA, and IC 50 values are indicated at the top of each plot. The experiments were performed in triplicate, and the data are presented as mean ± SEM and are representative of four ( A ) and two ( B ) independent experiments. ns, not significant; * P < 0.05, ** P < 0.01, **** P < 0.0001 (two-way ANOVA).

Human Intestinal Epithelial Cell Lines Caco 2 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 16805 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "A small-molecule HSP90 inhibitor, NVP-HSP990, alleviates rotavirus infection"

Article Title: A small-molecule HSP90 inhibitor, NVP-HSP990, alleviates rotavirus infection

Journal: Journal of Virology

doi: 10.1128/jvi.01883-25

Cytotoxicity and inhibitory effects on RV replication of different HSP90 inhibitors. ( A ) Plots for cell viability of MA104, Caco-2, and HT-29 cells after treatment with NVP-HSP990, GA, or 17-AAG at indicated concentrations for 24 h. Cell viability was tested using the CCK-8 assay. ( B ) Plots for RV (Wa and SA11 strains) inhibition in MA104, Caco-2, and HT-29 cells after treatment with NVP-HSP990, GA, or 17-AAG at indicated concentrations for 24 h. RV replication was tested by PFA, and IC 50 values are indicated at the top of each plot. The experiments were performed in triplicate, and the data are presented as mean ± SEM and are representative of four ( A ) and two ( B ) independent experiments. ns, not significant; * P < 0.05, ** P < 0.01, **** P < 0.0001 (two-way ANOVA).

Figure Legend Snippet: Cytotoxicity and inhibitory effects on RV replication of different HSP90 inhibitors. ( A ) Plots for cell viability of MA104, Caco-2, and HT-29 cells after treatment with NVP-HSP990, GA, or 17-AAG at indicated concentrations for 24 h. Cell viability was tested using the CCK-8 assay. ( B ) Plots for RV (Wa and SA11 strains) inhibition in MA104, Caco-2, and HT-29 cells after treatment with NVP-HSP990, GA, or 17-AAG at indicated concentrations for 24 h. RV replication was tested by PFA, and IC 50 values are indicated at the top of each plot. The experiments were performed in triplicate, and the data are presented as mean ± SEM and are representative of four ( A ) and two ( B ) independent experiments. ns, not significant; * P < 0.05, ** P < 0.01, **** P < 0.0001 (two-way ANOVA).

Techniques Used: CCK-8 Assay, Inhibition

NVP-HSP990 significantly inhibited RV replication, viral gene transcription, and antigen expression. ( A ) Caco-2 cells were infected with RV (Wa or SA11 strains) at varying MOIs (0.001–10), followed by treatment with 100 nM NVP-HSP990 or an equal volume of DMSO (as a control) for 24 h. Viral replication was assessed using PFA. ( B ) Schematic diagram illustrating the timing of NVP-HSP990 addition and removal. ( C ) Caco-2 cells were infected with RV (MOI = 1) and treated with 100 nM NVP-HSP990 or with DMSO as control at indicated infection periods as shown in panel B. Viral replication was tested with PFA at 20 h p.i. ( D ) Caco-2 cells were infected with RV (MOI = 1) and treated with 100 nM HSP990 at 0, 4, 8, 12 h p.i. or treated with DMSO at 0 h p.i. Virus replication was tested with PFA at 24 h p.i. ( E ) Caco-2 cells were infected with RV (MOI = 1) and further cultivated with DMEM containing 100 nM NVP-HSP990 or DMSO as control for 18 h. Then the infected cells were harvested for qPCR analysis of the expression of RV genes encoding the VP2, VP6, NSP4, and NSP5 proteins. ( F ) Caco-2 cells were mock-infected with PBS or infected with RV (MOI = 1) and further cultivated with DMEM containing 100 nM NVP-HSP990 or DMSO as control for 18 h. Then the cells were harvested for WB analysis of RV structural proteins VP6 and VP7. ( G ) Caco-2 cells growing on coverslips were mock-infected with PBS or infected with RV (MOI = 3) and treated with 100 nM NVP-HSP990 or DMSO as control for 18 h. Then the infected cells were subjected to immunostaining of RV VP6 antigens (red). Data are presented as mean ± SEM ( A, C, D, E ). The experiments were performed in triplicate ( A, C, D, E ), and the data are representative of two independent experiments ( A, C–G ). ns, not significant; ** P < 0.01, *** P < 0.001, **** P < 0.0001 (two-way ANOVA [ A, C, E ] and one-way ANOVA [ D ]).

Figure Legend Snippet: NVP-HSP990 significantly inhibited RV replication, viral gene transcription, and antigen expression. ( A ) Caco-2 cells were infected with RV (Wa or SA11 strains) at varying MOIs (0.001–10), followed by treatment with 100 nM NVP-HSP990 or an equal volume of DMSO (as a control) for 24 h. Viral replication was assessed using PFA. ( B ) Schematic diagram illustrating the timing of NVP-HSP990 addition and removal. ( C ) Caco-2 cells were infected with RV (MOI = 1) and treated with 100 nM NVP-HSP990 or with DMSO as control at indicated infection periods as shown in panel B. Viral replication was tested with PFA at 20 h p.i. ( D ) Caco-2 cells were infected with RV (MOI = 1) and treated with 100 nM HSP990 at 0, 4, 8, 12 h p.i. or treated with DMSO at 0 h p.i. Virus replication was tested with PFA at 24 h p.i. ( E ) Caco-2 cells were infected with RV (MOI = 1) and further cultivated with DMEM containing 100 nM NVP-HSP990 or DMSO as control for 18 h. Then the infected cells were harvested for qPCR analysis of the expression of RV genes encoding the VP2, VP6, NSP4, and NSP5 proteins. ( F ) Caco-2 cells were mock-infected with PBS or infected with RV (MOI = 1) and further cultivated with DMEM containing 100 nM NVP-HSP990 or DMSO as control for 18 h. Then the cells were harvested for WB analysis of RV structural proteins VP6 and VP7. ( G ) Caco-2 cells growing on coverslips were mock-infected with PBS or infected with RV (MOI = 3) and treated with 100 nM NVP-HSP990 or DMSO as control for 18 h. Then the infected cells were subjected to immunostaining of RV VP6 antigens (red). Data are presented as mean ± SEM ( A, C, D, E ). The experiments were performed in triplicate ( A, C, D, E ), and the data are representative of two independent experiments ( A, C–G ). ns, not significant; ** P < 0.01, *** P < 0.001, **** P < 0.0001 (two-way ANOVA [ A, C, E ] and one-way ANOVA [ D ]).

Techniques Used: Expressing, Infection, Control, Virus, Immunostaining

NVP-HSP990 alters the life state of host cells. Caco-2 cells were mock-infected with PBS or infected with RV Wa or SA11 strains (MOI = 3) and further cultivated with DMEM containing 100 nM NVP-HSP990 or an equal volume of DMSO as a control for 24 h. Then, the infected cells were harvested for RNA-seq analysis. ( A ) Multiple differential scatter plots of compared groups. ( B ) Venn diagrams of up- and downregulated genes between compared groups. ( C, D ) Top 10 (ranked by descending Q value) upregulated ( C ) and downregulated ( D ) KEGG pathways in all mock-, Wa-, and SA11-infected Caco-2 cells. ( E, F ) Top 10 (ranked by descending Q value) upregulated ( E ) and downregulated ( F ) KEGG pathways in both Wa- and SA11-infected but not in mock-infected Caco-2 cells. *Q value <0.05.

Figure Legend Snippet: NVP-HSP990 alters the life state of host cells. Caco-2 cells were mock-infected with PBS or infected with RV Wa or SA11 strains (MOI = 3) and further cultivated with DMEM containing 100 nM NVP-HSP990 or an equal volume of DMSO as a control for 24 h. Then, the infected cells were harvested for RNA-seq analysis. ( A ) Multiple differential scatter plots of compared groups. ( B ) Venn diagrams of up- and downregulated genes between compared groups. ( C, D ) Top 10 (ranked by descending Q value) upregulated ( C ) and downregulated ( D ) KEGG pathways in all mock-, Wa-, and SA11-infected Caco-2 cells. ( E, F ) Top 10 (ranked by descending Q value) upregulated ( E ) and downregulated ( F ) KEGG pathways in both Wa- and SA11-infected but not in mock-infected Caco-2 cells. *Q value <0.05.

Techniques Used: Infection, Control, RNA Sequencing

NVP-HSP990 inhibited MAPK activation and facilitated expression of tight junction-associated proteins in intestinal cells. ( A ) MA104, Caco-2, and HT-29 cells were mock-infected with PBS or infected with RV Wa or SA11 strains (MOI = 3), followed by treatment of 100 nM HSP990 (+) or an equal volume of DMSO as a control (−) for 20 h. Then the infected cells were harvested for WB analysis of MAPK components. ( B ) Caco-2 cells were mock-infected with PBS, treated with 1 µM C16-PAF(C16), or infected with RV Wa or SA11 strains (MOI = 3), and then treated with 100 nM HSP990 (+) or DMSO as a control (−) for 20 h. The infected cells were harvested for WB analysis of MAPK components. ( C ) Caco-2 cells were mock-infected with PBS or infected with RV Wa or SA11 strains (MOI = 3), and then treated with 100 nM HSP990 (+) or DMSO as control (−) for 20 h. Then the infected cells were harvested for WB analysis of tight junction-associated proteins. Data are representative of three ( A ) and two ( B and C ) independent experiments.

Figure Legend Snippet: NVP-HSP990 inhibited MAPK activation and facilitated expression of tight junction-associated proteins in intestinal cells. ( A ) MA104, Caco-2, and HT-29 cells were mock-infected with PBS or infected with RV Wa or SA11 strains (MOI = 3), followed by treatment of 100 nM HSP990 (+) or an equal volume of DMSO as a control (−) for 20 h. Then the infected cells were harvested for WB analysis of MAPK components. ( B ) Caco-2 cells were mock-infected with PBS, treated with 1 µM C16-PAF(C16), or infected with RV Wa or SA11 strains (MOI = 3), and then treated with 100 nM HSP990 (+) or DMSO as a control (−) for 20 h. The infected cells were harvested for WB analysis of MAPK components. ( C ) Caco-2 cells were mock-infected with PBS or infected with RV Wa or SA11 strains (MOI = 3), and then treated with 100 nM HSP990 (+) or DMSO as control (−) for 20 h. Then the infected cells were harvested for WB analysis of tight junction-associated proteins. Data are representative of three ( A ) and two ( B and C ) independent experiments.

Techniques Used: Activation Assay, Expressing, Infection, Control

NVP-HSP990 mitigated disruption of tight junctions in RV infection. Caco-2 cells growing on coverslips were mock-infected or infected with RV Wa or SA11 strains (MOI = 3), and cultivated with DMEM containing 100 nM NVP-HSP990 or an equal volume of DMSO as a control after infection for another 18 h. Then the infected cells were applied for immunostaining for RV antigens (green), ZO-1 (red), and DAPI staining of nucleus (blue). Data are representative of two independent experiments.

Figure Legend Snippet: NVP-HSP990 mitigated disruption of tight junctions in RV infection. Caco-2 cells growing on coverslips were mock-infected or infected with RV Wa or SA11 strains (MOI = 3), and cultivated with DMEM containing 100 nM NVP-HSP990 or an equal volume of DMSO as a control after infection for another 18 h. Then the infected cells were applied for immunostaining for RV antigens (green), ZO-1 (red), and DAPI staining of nucleus (blue). Data are representative of two independent experiments.

Techniques Used: Disruption, Infection, Control, Immunostaining, Staining

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Journal: Journal of Virology

Article Title: A small-molecule HSP90 inhibitor, NVP-HSP990, alleviates rotavirus infection

doi: 10.1128/jvi.01883-25

Figure Lengend Snippet: Cytotoxicity and inhibitory effects on RV replication of different HSP90 inhibitors. ( A ) Plots for cell viability of MA104, Caco-2, and HT-29 cells after treatment with NVP-HSP990, GA, or 17-AAG at indicated concentrations for 24 h. Cell viability was tested using the CCK-8 assay. ( B ) Plots for RV (Wa and SA11 strains) inhibition in MA104, Caco-2, and HT-29 cells after treatment with NVP-HSP990, GA, or 17-AAG at indicated concentrations for 24 h. RV replication was tested by PFA, and IC 50 values are indicated at the top of each plot. The experiments were performed in triplicate, and the data are presented as mean ± SEM and are representative of four ( A ) and two ( B ) independent experiments. ns, not significant; * P < 0.05, ** P < 0.01, **** P < 0.0001 (two-way ANOVA).

Article Snippet: Human intestinal epithelial cell lines Caco-2 cells (ATCC: HTB-37) and HT-29 cells (ATCC: HTB-38) were from ATCC and kept in our institute.

Techniques: CCK-8 Assay, Inhibition

Journal: Journal of Virology

Article Title: A small-molecule HSP90 inhibitor, NVP-HSP990, alleviates rotavirus infection

doi: 10.1128/jvi.01883-25

Figure Lengend Snippet: NVP-HSP990 significantly inhibited RV replication, viral gene transcription, and antigen expression. ( A ) Caco-2 cells were infected with RV (Wa or SA11 strains) at varying MOIs (0.001–10), followed by treatment with 100 nM NVP-HSP990 or an equal volume of DMSO (as a control) for 24 h. Viral replication was assessed using PFA. ( B ) Schematic diagram illustrating the timing of NVP-HSP990 addition and removal. ( C ) Caco-2 cells were infected with RV (MOI = 1) and treated with 100 nM NVP-HSP990 or with DMSO as control at indicated infection periods as shown in panel B. Viral replication was tested with PFA at 20 h p.i. ( D ) Caco-2 cells were infected with RV (MOI = 1) and treated with 100 nM HSP990 at 0, 4, 8, 12 h p.i. or treated with DMSO at 0 h p.i. Virus replication was tested with PFA at 24 h p.i. ( E ) Caco-2 cells were infected with RV (MOI = 1) and further cultivated with DMEM containing 100 nM NVP-HSP990 or DMSO as control for 18 h. Then the infected cells were harvested for qPCR analysis of the expression of RV genes encoding the VP2, VP6, NSP4, and NSP5 proteins. ( F ) Caco-2 cells were mock-infected with PBS or infected with RV (MOI = 1) and further cultivated with DMEM containing 100 nM NVP-HSP990 or DMSO as control for 18 h. Then the cells were harvested for WB analysis of RV structural proteins VP6 and VP7. ( G ) Caco-2 cells growing on coverslips were mock-infected with PBS or infected with RV (MOI = 3) and treated with 100 nM NVP-HSP990 or DMSO as control for 18 h. Then the infected cells were subjected to immunostaining of RV VP6 antigens (red). Data are presented as mean ± SEM ( A, C, D, E ). The experiments were performed in triplicate ( A, C, D, E ), and the data are representative of two independent experiments ( A, C–G ). ns, not significant; ** P < 0.01, *** P < 0.001, **** P < 0.0001 (two-way ANOVA [ A, C, E ] and one-way ANOVA [ D ]).

Article Snippet: Human intestinal epithelial cell lines Caco-2 cells (ATCC: HTB-37) and HT-29 cells (ATCC: HTB-38) were from ATCC and kept in our institute.

Techniques: Expressing, Infection, Control, Virus, Immunostaining

Journal: Journal of Virology

Article Title: A small-molecule HSP90 inhibitor, NVP-HSP990, alleviates rotavirus infection

doi: 10.1128/jvi.01883-25

Figure Lengend Snippet: NVP-HSP990 alters the life state of host cells. Caco-2 cells were mock-infected with PBS or infected with RV Wa or SA11 strains (MOI = 3) and further cultivated with DMEM containing 100 nM NVP-HSP990 or an equal volume of DMSO as a control for 24 h. Then, the infected cells were harvested for RNA-seq analysis. ( A ) Multiple differential scatter plots of compared groups. ( B ) Venn diagrams of up- and downregulated genes between compared groups. ( C, D ) Top 10 (ranked by descending Q value) upregulated ( C ) and downregulated ( D ) KEGG pathways in all mock-, Wa-, and SA11-infected Caco-2 cells. ( E, F ) Top 10 (ranked by descending Q value) upregulated ( E ) and downregulated ( F ) KEGG pathways in both Wa- and SA11-infected but not in mock-infected Caco-2 cells. *Q value <0.05.

Article Snippet: Human intestinal epithelial cell lines Caco-2 cells (ATCC: HTB-37) and HT-29 cells (ATCC: HTB-38) were from ATCC and kept in our institute.

Techniques: Infection, Control, RNA Sequencing

Journal: Journal of Virology

Article Title: A small-molecule HSP90 inhibitor, NVP-HSP990, alleviates rotavirus infection

doi: 10.1128/jvi.01883-25

Figure Lengend Snippet: NVP-HSP990 inhibited MAPK activation and facilitated expression of tight junction-associated proteins in intestinal cells. ( A ) MA104, Caco-2, and HT-29 cells were mock-infected with PBS or infected with RV Wa or SA11 strains (MOI = 3), followed by treatment of 100 nM HSP990 (+) or an equal volume of DMSO as a control (−) for 20 h. Then the infected cells were harvested for WB analysis of MAPK components. ( B ) Caco-2 cells were mock-infected with PBS, treated with 1 µM C16-PAF(C16), or infected with RV Wa or SA11 strains (MOI = 3), and then treated with 100 nM HSP990 (+) or DMSO as a control (−) for 20 h. The infected cells were harvested for WB analysis of MAPK components. ( C ) Caco-2 cells were mock-infected with PBS or infected with RV Wa or SA11 strains (MOI = 3), and then treated with 100 nM HSP990 (+) or DMSO as control (−) for 20 h. Then the infected cells were harvested for WB analysis of tight junction-associated proteins. Data are representative of three ( A ) and two ( B and C ) independent experiments.

Article Snippet: Human intestinal epithelial cell lines Caco-2 cells (ATCC: HTB-37) and HT-29 cells (ATCC: HTB-38) were from ATCC and kept in our institute.

Techniques: Activation Assay, Expressing, Infection, Control

Journal: Journal of Virology

Article Title: A small-molecule HSP90 inhibitor, NVP-HSP990, alleviates rotavirus infection

doi: 10.1128/jvi.01883-25

Figure Lengend Snippet: NVP-HSP990 mitigated disruption of tight junctions in RV infection. Caco-2 cells growing on coverslips were mock-infected or infected with RV Wa or SA11 strains (MOI = 3), and cultivated with DMEM containing 100 nM NVP-HSP990 or an equal volume of DMSO as a control after infection for another 18 h. Then the infected cells were applied for immunostaining for RV antigens (green), ZO-1 (red), and DAPI staining of nucleus (blue). Data are representative of two independent experiments.

Article Snippet: Human intestinal epithelial cell lines Caco-2 cells (ATCC: HTB-37) and HT-29 cells (ATCC: HTB-38) were from ATCC and kept in our institute.

Techniques: Disruption, Infection, Control, Immunostaining, Staining

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